Control of American Foulbrood by the Shaking Method

نویسندگان

  • Henrik Hansen
  • Camilla J. Brødsgaard
چکیده

In a Danish apiary 15 bee colonies were fed honey containing 1.0×10 P. l. larvae spores and examined for two beekeeping seasons. 43 days after the spore feeding, 12 of the colonies showed clinical symptoms of American foulbrood (AFB). Only these 12 colonies were then treated with the shaking method. After the shaking method was carried out a major reduction in the number of spores in the honey of the treated colonies was seen. The number of spores were reduced to a level at which they did not provoke further clinical symptoms of AFB. Even though all colonies were fed the same number of spores 20% of the colonies never showed clinical symptoms. These colonies were able to reduce the number of spores in the honey within one season to a level that was equal to the treated colonies. In Denmark, the shaking method has been used successfully for the past 90 years.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of PCR method for diagnosing of honey bee American Foulbrood disease

American foulbrood (AFB) disease is caused by the sporeforming bacterium Paenibacillus larvae larvae. Traditional diagnosis is based on culture technique is time and laboratory work consuming. In this study with standard strain, PCR was developed by specific primers and PCR products were electrophoresed on 0.8 % agarose gel. The PCR primers were selected on the basis of the 16S rRNA gene and am...

متن کامل

Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

متن کامل

Investigation of Paenibacillus larvae subspecies larva of honey bee (Apis mellifera) colonies in East Azerbaijan Province of Iran

Background: The American foulbrood disease (AFB), caused by Paenibacillus larvae subsp. larvae, is one of the main plagues affecting honey bee (Apis mellifera L.) colonies, and it has a high negative impact on beekeepers worldwide. OBJECTIVES: The aim of this research was to describe the evidence of Paenibacillus larvae larvae contamination of honey bee colonies, in the East Azerbaijan Province...

متن کامل

Control of American Foulbrood by using alternatively eradication method and artificial swarms

Introduction American Foulbrood (AFB) is one of the most threatening epidemic brood diseases in honey bee colonies. This disease is caused by the pathogen Paenibacillus larvae larvae. According to the common animal health law in Germany the outbreak of AFB has to be reported to veterinarian service. The veterinarian has to decide about eradication of bee colonies, cleaning and disinfection as w...

متن کامل

American Foulbrood in honeybees and its causative agent, Paenibacillus larvae.

After more than a century of American Foulbrood (AFB) research, this fatal brood infection is still among the most deleterious bee diseases. Its etiological agent is the Gram-positive, spore-forming bacterium Paenibacillus larvae. Huge progress has been made, especially in the last 20 years, in the understanding of the disease and of the underlying host-pathogen interactions. This review will p...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003